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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may well contribute to tumorigenesis. This protein was discovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital function in endorsing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two more splice variants have been documented but in-depth nucleotide sequence info is just not nevertheless out there.
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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide usually includes the epitope acknowledged from the antibody.
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This manual outlines further more probable optimization of mobile and immunoassay parameters to be sure the absolute best final results are...
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This information outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
Antibodies sure to the blocking peptide not bind to the epitope to the target protein. By evaluating the staining in the blocked antibody vs . the antibody on your own, you can see which staining is certain.
Target Facts Cyclin E belongs for the extremely conserved cyclin relatives, whose members are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation patterns which contribute to your temporal coordination of each mitotic function. Cyclin E forms a fancy with and functions as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates in the G1-S stage boundary which is degraded as cells development by way of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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